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anti clic3  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti clic3
    Anti Clic3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+clic3/pmc12433953-77-37-39
    Average 86 stars, based on 1 article reviews
    anti clic3 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Single-cell RNA-seq reveals a repair pattern in cystic lesions in steroid induced osteonecrosis of the femoral head
    Article Snippet: Samples (30 μg/lane) were resolved on 10% SDS-PAGE gels and transferred to PVDF membranes (Beyotime, FFP20). .. After blocking with 5% non-fat milk (Beyotime, P0216) in TBST (1 h, RT), membranes were incubated overnight at 4°C with primary antibodies: anti-SPP1 (1:1000; Abcam, ab58632), anti-RUNX2 (1:800; CST, #94808), anti-ColoA1 (1:800; CST, #94808), anti-MMP3(1:800; CST, #94808), anti-CLIC3 (1:800; CST, #94808), anti-GAPDH (1:5000; Proteintech, 60004-1-Ig). ..

    Incubation:

    Article Title: Single-cell RNA-seq reveals a repair pattern in cystic lesions in steroid induced osteonecrosis of the femoral head
    Article Snippet: Samples (30 μg/lane) were resolved on 10% SDS-PAGE gels and transferred to PVDF membranes (Beyotime, FFP20). .. After blocking with 5% non-fat milk (Beyotime, P0216) in TBST (1 h, RT), membranes were incubated overnight at 4°C with primary antibodies: anti-SPP1 (1:1000; Abcam, ab58632), anti-RUNX2 (1:800; CST, #94808), anti-ColoA1 (1:800; CST, #94808), anti-MMP3(1:800; CST, #94808), anti-CLIC3 (1:800; CST, #94808), anti-GAPDH (1:5000; Proteintech, 60004-1-Ig). ..



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    Image Search Results


    Antibodies Used in the Experiment

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Expression Distribution of Keratins in Normal and Pathological Corneas and the Regulatory Role of Krt17 on Limbal Stem Cells

    doi: 10.1167/iovs.66.4.55

    Figure Lengend Snippet: Antibodies Used in the Experiment

    Article Snippet: Clic3 , Proteintech , 15971-1-AP.

    Techniques:

    Knockdown of Krt17 in HLECs downregulates epithelial development and differentiation. ( A ) GO analysis of the biological process of DEGs after Krt17-specific siRNA treatment revealed that downregulated genes are enriched in epithelial development and differentiation pathways. ( B ) The heatmap displays representative DEGs associated with corneal epithelial differentiation. ( C ) Prediction of mouse KRT17 interacting proteins. ( D – F ) Krt17 knockdown leads to downregulation of the corneal epithelial stem cell marker Krt14, as well as two other stress KRTs, Krt16 and Krt6a ( n = 4/group). ( G – L ) Krt17 knockdown significantly suppresses the mRNA levels of corneal epithelial differentiation markers Clu, Ivl, Aldh3a1, Krt3, Clic3, and Hopx ( n = 4/group). ( M ) Krt17 knockdown in HLECs inhibits the protein expression of Krt3, Clic3 and Hopx. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Expression Distribution of Keratins in Normal and Pathological Corneas and the Regulatory Role of Krt17 on Limbal Stem Cells

    doi: 10.1167/iovs.66.4.55

    Figure Lengend Snippet: Knockdown of Krt17 in HLECs downregulates epithelial development and differentiation. ( A ) GO analysis of the biological process of DEGs after Krt17-specific siRNA treatment revealed that downregulated genes are enriched in epithelial development and differentiation pathways. ( B ) The heatmap displays representative DEGs associated with corneal epithelial differentiation. ( C ) Prediction of mouse KRT17 interacting proteins. ( D – F ) Krt17 knockdown leads to downregulation of the corneal epithelial stem cell marker Krt14, as well as two other stress KRTs, Krt16 and Krt6a ( n = 4/group). ( G – L ) Krt17 knockdown significantly suppresses the mRNA levels of corneal epithelial differentiation markers Clu, Ivl, Aldh3a1, Krt3, Clic3, and Hopx ( n = 4/group). ( M ) Krt17 knockdown in HLECs inhibits the protein expression of Krt3, Clic3 and Hopx. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: Clic3 , Proteintech , 15971-1-AP.

    Techniques: Knockdown, Marker, Expressing

    Human Primer Sequences Used for qPCR

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Expression Distribution of Keratins in Normal and Pathological Corneas and the Regulatory Role of Krt17 on Limbal Stem Cells

    doi: 10.1167/iovs.66.4.55

    Figure Lengend Snippet: Human Primer Sequences Used for qPCR

    Article Snippet: Clic3 , Proteintech , 15971-1-AP.

    Techniques: